Volume 14 - 2023 - Issue 2: SI Recent Advances in Colletotrichum Taxonomy & Sys

1. Genome-scale and multi-gene phylogenetic analyses of Colletotrichum spp. host preference and associated with medicinal plants

Authors: Zhang Q, Nizamani MM, Feng Y, Yang YQ, Jayawardena RS, Hyde KD, Wang Y, Li C

Recieved: 18 November 2022, Accepted: 22 February 2023, Published: 23 March 2023

Colletotrichum is one of the most common phytopathogens worldwide, causing diseases in various commercially valuable hosts. Several Colletotrichum species have been reported to infect medicinal plants. Although China is a large-scale producer of medicinal plant resources, the species of Colletotrichum that have been locally linked to medicinal plants are poorly understood. Therefore, samples were collected from four provinces of China to determine the Colletotrichum species associated with local medicinal plants, resulting in a total of 141 Colletotrichum isolates. In conjunction with morphology, multi-locus phylogenetic analyses (ITS, gapdh, chs-1, his3, act, and tub2), and PHI test revealed that these strains belong to 23 species in eight species complexes, including seven new species (C. castaneae, C. cypericola, C. gardeniae, C. kunmingense, C. ligustri, C. radermacherae, and C. schefflerae) and three new host records (C. celtidis, C. iris, and C. vittalense). In addition, we synonymise C. wuxuhaiense to C. karsti, and C. menglaense, C. pandanicola, and C. parvisporum to C. siamense. Furthermore, to understand mechanisms responsible for host range and preference we also sequenced and assembled whole-genome sequences of four species in the C. gloeosporioides complex and constructed whole-genome phylogenies of Colletotrichum species covering ten species complexes and three singleton species. The comparative genome of Colletotrichum species from different lineages revealed that expanded gene families encoding CAZymes are thought to be one of the likely explanations for the widespread and polyphagous nature of species in the C. acutatum, C. boninense, and C. gloeosporioides species complexes. A possible explanation for the host preference of the C. graminicola species complex may be the less pectinase-encoding gene families. The current study improves the understanding of Colletotrichum species diversity found on medicinal plants and suggests that the members of CAZymes might serve as an important mechanism for host range and preference.

Keywords: Comparative genomics – Genome prediction – Multi-locus phylogeny – Phylogenomics – Seven new species


2. Identification and characterization of Colletotrichum species associated with durian fruit in northern Thailand

Authors: Armand A, Hyde KD, Huanraluek N, Wang Y, Jayawardena RS

Recieved: 13 January 2023, Accepted: 10 February 2023, Published: 23 March 2023

Colletotrichum is one of the most important plant pathogenic genera affecting different plant species, particularly tropical and sub-tropical crops and fruits. Species of the genus can cause many diseases, including fruit rots, crown rots, stem end rots, and anthracnose. The objective of the present study was to identify the Colletotrichum species associated with durian fruit rots in northern Thailand. Based on morphological study and phylogenetic analyses of five loci (internal transcribed spacer (ITS), actin (ACT), chitin synthase 1 (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and ß-tubulin (TUB2)), four species belonging to three complexes were identified and described. Colletotrichum durionigenum is introduced as a new species, and C. gigasporum, C. pandanicola, and C. truncatum are described and illustrated as new host records.

Keywords: 1 new species – Diversity – Durio spp. – Glomerellaceae – Phylogeny – Phytopathogen


3. Colletotrichum species associated with Camellia anthracnose in China

Authors: Peng XJ, Wang QC, Zhang SK, Guo K, Zhou XD

Recieved: 18 November 2022, Accepted: 02 May 2023, Published: 30 May 2023

Species of Camellia oleifera, Ca. sinensis and Ca. japonica represent globally-important tree germplasm resource and have significant ecological and economic value. However, they have suffered from anthracnose caused by pathogenic fungi from the genus of Colletotrichum. To determine the diversity of Colletotrichum species associated with Camellia anthracnose in China, we collected infected leaves from three major cultivation provinces. A total number of 167 fungal strains resembling Colletotrichum were obtained, 41 from Ca. oleifera, 48 from Ca. sinensis, and 78 from Ca. japonica. Comparison of morphology and phylogenetic analyses based on six loci (ACT, CAL, CHS-1, GAPDH, TUB2 and ITS) of the representative isolates revealed that they fall in four species complexes and represent 15 known and one undescribed taxa. The species complexes are Colletotrichum gloeosporioides (C. aenigma, C. alienum, C. camelliae, C. fructicola, C. gloeosporioides, C. jiangxiense, C. pandanicola, C. siamense, C. wuxiense and C. puerense described here), C. acutatum (C. fioriniae and C. nymphaeae), C. boninense (C. boninense and C. karstii) and C. orchidearum (C. clivicola and C. plurivorum). Of these, C. fructicola was the most dominant species occurring on Ca. oleifera (68.3%), Ca. sinensis (50.0%) and Ca. japonica (29.49%). Pathogenicity test results indicated that C. camelliae is the most pathogenic to the three Camellia species. This study represents the first report of C. aenigma, C. alienum, C. boninense, C. camelliae, C. clivicola, C. fioriniae, C. fructicola, C. gloeosporioides, C. jiangxinse, C. karstii, C. nymphaeae, C. pandanicola and C. wuxiense causing anthracnose on Ca. japonica and C. pandanicola on Ca. sinensis. Knowledge gained in this study enlarges our understanding of Colletotrichum species causing anthracnose on three important Camellia trees and contributes to the disease management.

Keywords: 1 new species – Camellia japonica – fungal diversity – multi-gene phylogeny – pathogenicity


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Mycosphere publishes reviews, research articles, methodology papers, taxonomic works such as monographs, which are relevant to fungal biology, including lichens. The official journal language is English.


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