Recent Papers

Volume 14 - 2023 Issue 1

2. Morphology and multigene phylogeny reveal ten novel taxa in Ascomycota from terrestrial palm substrates (Arecaceae) in Thailand
Konta S et al. (2023)

1. Endophytic fungi in green manure crops; friends or foe?
Abeywickrama PD et al. (2023)

Volume 13 - 2022 Issue 2 (SI Fungal Evolution)

9. Special Issue: Fungal Evolution, in honour of the Academician Professor Yu Li’s 80th Birthday
Hyde Kevin David et al. (2023)

8. Diversity, molecular dating and ancestral characters state reconstruction of entomopathogenic fungi in Hypocreales
Wei DP et al. (2022)

7. Evolutionary relationships and allied species of Pyronemataceae, with segregation of the novel family Pyropyxidaceae
Zeng M et al. (2022)

6. Fossil Tetraploa redefinition and potential contribution of dark pigments for the preservation of its spores in the fossil record
Nuñez Otaño NB et al. (2022)

5. Comparative genomics provides new insights into the evolution of Colletotrichum
Chen YP et al. (2022)

4. Large-scale genome investigations reveal insights into domestication of cultivated mushrooms
Fu YP et al. (2022)

3. Evolutionary relationship and a novel method of efficient identification of Lentinula edodes cultivars in China
Ling YY et al. (2022)

2. Phylogenetic diversity and affiliation of tropical African ectomycorrhizal fungi
Houdanon RD et al. (2022)

Volume 14 - 2023 - Issue 1

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1. Endophytic fungi in green manure crops; friends or foe?

Authors: Abeywickrama PD, Qian N, Jayawardena RS, Li Y, Zhang W, Guo K, Zhang L, Zhang G, Yan J, Li X, Guo Z, Hyde KD, Peng Y, Zhao W

Recieved: 03 April 2022, Accepted: 10 December 2022, Published: 13 January 2023

Astragalus sinicus and Vicia villosa; are frequently applied green manure plants used in China. However, there is poor knowledge of the fungal endophytic community and the mycobiome of green manure crops. Field surveys were conducted during 2017–2019 in four provinces in China. Asymptomatic plant parts were collected. Using a culture-dependent method, 517 fungal isolates were obtained from Astragalus sinicus and Vicia villosa. These isolates were further identified using a combination of morphological and multi-loci phylogenetic analyses and were differentiated into 30 species in 15 genera in ten families belonging to only Ascomycota. Most isolated strains belonged to Sordariomycetes. The most dominant genus was Fusarium, with 381 isolates from both crops, while all other taxa were isolated less than 40 times. The similarity search on the Fusarium MLST database showed the 370 strains belonged to seven Fusarium complexes and one subclade. Eleven strains could not be assigned to any complex. The remaining 136 isolates were identified and assigned to 23 known and seven novel species. A total of 178 Operational Taxonomic Units (OTUs) were obtained from Illumina analysis and mainly classified into five phyla (Ascomycota, Basidiomycota, Chytridiomycota, Cryptomycota, and Mucoromycota). Overall OTUs were further assigned to 21 classes, 48 orders, 66 families, and 74 genera. Based on overall OTUs, the most abundant species was Alternaria alternata, which was also isolated from the culture-dependent method. Most species and genera recorded from the High Throughput Sequencing (HTS) approach were not obtained in the culture-dependent method (Boeremia, Cladosporium, Filobasidium, Magnoporthe, Mucor, Rhizoctonia, Sporidiobolus). Functional annotation reveals that all Ascomycetes genera obtained in both approaches comprised several plant pathogenic species. Potential beneficial and/or biocontrol strains were also identified. The common green manure crops used in China harbors a hidden, underexplored mycobiome which may comprise potential for application. These results will increase awareness of green manure practices. Precautions need to be in place when incorporating green manure crops in the soil, as these could facilitate inoculum sources for the next disease cycle of the main crop.

Keywords: 7 new taxa – Checklist – Culture dependent – Cover crops – High-throughput sequencing – Taxonomy

 

2. Morphology and multigene phylogeny reveal ten novel taxa in Ascomycota from terrestrial palm substrates (Arecaceae) in Thailand

Authors: Konta S, Tibpromma S, Karunarathna SC, Samarakoon MC, Steven LS, Mapook A, Boonmee S, Senwanna C, Balasuriya A, Eungwanichayapant PD, Hyde KD

Recieved: 11 August 2022, Accepted: 28 November 2022, Published: 13 January 2023

Arecaceae is one of the important plant families in Thailand, and several of its representative genera are used for food, cosmetics, and energy sources. During the past eight years (2014 to 2022), more than 50 new taxa associated with terrestrial palm substrates have been reported in Thailand. The present study continued the survey of fungi associated with terrestrial palms in Thailand and based on both morphological characteristics and phylogenetic analyses of combined sequence data, fungal taxa belonging to the orders Amphisphaeriales, Diaporthales, Distoseptisporales, Glomerellales, Pleosporales, and Xylariales were identified. A new genus Triseptatospora and nine new species viz., Acremoniisimulans cocois, Acrocalymma arengae, Bartalinia adonidiae, Cytospora calamicola, Distoseptispora licualae, Neopestalotiopsis elaeidis, Pteridiospora arengae, Triseptatospora calami, and Xenoanthostomella calami are described and introduced here. Morphological illustrations, descriptions, and phylogenetic trees which indicate the placement of the new taxa are provided.

Keywords: 10 new taxa – Dothideomycetes – palm fungi – phylogeny – saprobe – Sordariomycetes – taxonomy – Thai fungi