Recent Papers

Volume 7 - 2016 - Issue 9 (SI Tribute to Prof. Jones)

1. A tribute to Professor E.B. Gareth Jones on his 80th birthday

Authors: Chomnunti S, Boonmee S, Hongsanan S, Doilom M, Thongklang N, Wanasinghe DN, Suetrong S

Recieved: 30 November -0001, Accepted: 30 November -0001, Published: 25 December 2016

Keywords:

2. Parafuscosporella garethii sp. nov. (Fuscosporellales) from a community northern forest, in Thailand

Authors: Boonyuen N¹, Chuaseeharonnachai C1, Suetrong S1, Sujinda S1 and Somrithipol S1

Recieved: 25 October 2016, Accepted: 04 December 2016, Published: 28 December 2016

Parafuscosporella garethii, a new freshwater taxon, is described and illustrated from submerged decaying twigs in a Thai community forest located in Chiang Mai Province. The species is recognized as the third species in the genus, and markedly differs from those previously accepted in the genus by its conidial size and shape and having two forms of conidiogenous cells. Phylogenetic analysis based on combined LSU, SSU and RPB2 sequence data place P. garethii in Fuscosporellales, Hypocreomycetidae, Sordariomycetes. The novel taxon is compared with morphologically and phylogenetically similar species in the genus and a taxonomic comparison to accepted Parafuscosporella species is also provided

Keywords: E.B. Gareth Jones – freshwater fungi – Fuscosporellaceae – phylogeny – taxonomy

3. Stachybotrys-like taxa from karst areas and a checklist of stachybotrys-like species from Thailan

Authors: Lin CG, McKenzie EHC, Bhat DJ, Ran SF, Chen Y, Hyde KD, Li DW, Wang

Recieved: 15 November 2016, Accepted: 02 December 2016, Published: 28 December 2016

During a survey of hyphomycetes from karst areas in Thailand, four stachybotrys-like taxa, viz., Cymostachys garethjonesii sp. nov., Memnoniella oblongispora sp. nov., M. nilagirica comb. nov. and Stachybotrys microspora were identified and are provided with descriptions in this paper. The new species are introduced based on morphological and molecular differences and compared with similar or related taxa. Memnoniella nilagirica and Stachybotrys microspora are new records for Thailand. An annotated checklist of stachybotrys-like taxa in Thailand is provided based on previous publications and database searches

Keywords: karst – new species – phylogeny – Stachybotryaceae – taxonomy

4. Venturia species form sooty mold-like colonies on leaves of Salix: introducing Venturia fuliginosa sp. nov

Authors: M. Shen, J.Q. Zhang, Y. Zhang

Recieved: 02 December 2016, Accepted: 27 December 2016, Published: 28 December 2016

Collection of ascomycetes in the Lesser Khingan Mountains of China led to the discovery of two species of Venturia on Salix, one of which is described and illustrated as a new species－V. fuliginosa in this paper. Venturia fuliginosa forms a sooty mold-like phenotype on the leaf surface of the host, and differs from known species of Venturia in having fusiform, subcylindrical, sometimes obpyriform and mostly aseptate conidia. Phylogenetic inference based on analysis of combined LSU and ITS sequence data, indicate that the new species is closely related to a strain named V. chlorospora (CBS 470.61) isolated from Salix daphnoides in 1958 in France. The second species, Venturia catenospora, is reported for the first time in China.

Keywords: follicolous fungi – phytopathogen – sooty molds – taxonomy

5. New species of Thozetella and Chaetosphaeria and new records of Chaetosphaeria and Tainosphaeria from Thailand

Authors: Perera RH, Maharachchikumbura SSN, Bhat JD, Al-Sadi AM, Liu JK, Hyde KD, Liu ZY

Recieved: 20 October 2016, Accepted: 04 December 2016, Published: 28 December 2016

We are studying seed and fruit-borne fungi in Thailand and in this paper report on species of Tainosphaeria, Thozetella and Chaetosphaeria from Fabaceae seed pods and decorticated wood, collected in Chiang Mai and Phang-nga provinces. Phylogenetic analysis of combined LSU, ITS and TUB sequence data provides evidence for new species of Thozetella and Chaetosphaeria. These new taxa are introduced and compared with closely related species in these genera. We provide a morphological and illustrated account of Chaetosphaeria panamensis isolated from Pinus twigs as a first record for Thailand.

Keywords: Chaetosphaeriales – morphology – phylogeny – seed/fruit fungi – Sordariomycetes

6. Brunneodinemasporium jonesii and Tainosphaeria jonesii spp. nov. (Chaetosphaeriaceae, Chaetosphaeriales) from southern China.

Authors: Lu YZ, Liu JK, Hyde KD, Bhat DJ, Xiao YP, Tian Q, Wen TC, Boonmee S, Kang JC

Recieved: 27 October 2016, Accepted: 10 December 2016, Published: 28 December 2016

A study of the fungi of Guangxi Province, China revealed two novel species, namely Brunneodinemasporium jonesii and Tainosphaeria jonesii which are introduced in this paper. Molecular analysis based on combined ITS and LSU sequence data showed that 1) Brunneodinemasporium jonesii formed a distinct clade with the type species B. brasiliense and is therefore introduced as the second species in this monotypic genus; 2) The isolates of Tainosphaeria jonesii clustered together with T. crassiparies and T. siamensis, and showed a close relationship with T. siamensis, but is a phylogenetically distinct species. It is herein described as the third species of Tainosphaeria. Morphological examination showed that 1) Brunneodinemasporium jonesii differs from the generic type species as its conidia have mucilaginous balls at each end, which are aggregated in chains, arising from conidiogenous cells that are aggregated into conspicuous slimy, dome-shaped masses; 2) Tainosphaeria jonesii is characterized by its mononematous conidiophores, phialidic conidiogenous cells with funnel-shaped collarettes at the apices and conidia with single unbranched, flexuous, tubular appendages at each end. Descriptions, illustrations and molecular analyses are provided for these new species.

Keywords: New species – phylogeny – Sordariomycetes – taxonomy – woody fungi

7. Two new species of Arthrinium (Apiosporaceae, Xylariales) associated with bamboo from Yunnan, China

Authors: Dai DQ, Jiang HB, Tang LZ, Bhat DJ

Recieved: 20 October 2016, Accepted: 04 December 2016, Published: 28 December 2016

Arthrinium, a globally distributed genus, is characterized by basauxic conidiogenesis in its asexual morph, with globose to subglobose conidia, which are usually lenticular in side view and obovioid and brown to dark brown. The sexual morph develops multi-locular perithecial stromata with hyaline apiospores usually surrounded by a thick gelatinous sheath. Four Arthrinium species collected in Kunming, China, are described and illustrated in this paper. Based on the morphology and analyses of ITS sequence data, we introduce two new species of Arthrinium. Arthrinium garethjonesii and A. neosubglobosa spp. nov. are provided with descriptions of sexual morphs and compared with phylogenetically close taxa. Arthrinium hydei and A. hyphopodii are new records on their sexual morphs.

Keywords: bambusicolous fungi – phylogeny – Sordariomycetes – taxonomy

8. Taxonomy and phylogenetic appraisal of Montagnula jonesii sp. nov. (Didymosphaeriaceae, Pleosporales).

Authors: Tennakoon DS, Hyde KD, Wanasinghe DN, Bahkali AH, Camporesi E, Khan S, Phookamsak R

Recieved: 01 November 2016, Accepted: 29 December 2016, Published: 28 December 2016

A saprobic member of Dothideomycetes was collected from dead branches of Fagus sylvatica in Italy. Morphology coupled with combined gene analysis of LSU, SSU, ITS and tef1-α sequence data, showed it to be a novel Montagnula species, which is introduced in this paper. Montagnula jonesii sp. nov. differs from other Montagnula species in having immersed, brown ascomata and ellipsoidal to fusiform, 3-septate ascospores with rounded ends and prominent guttules in each cell and is enlarged at the second cell from the apex. The new species is compared with other Montagnula species and a comprehensive description and micrographs are provided.

Keywords: Dothideomycetes – Morphology – New species

9. New species and records of Dictyocheirospora from submerged wood in north-western Yunnan, China

Authors: Wang RX, Luo ZL, Hyde KD, Bhat DJ, Su XJ, Su HY

Recieved: 25 November 2016, Accepted: 21 December 2016, Published: 28 December 2016

Two new species of Dictyocheirospora, D. aquatica and D. garethjonesii, isolated from submerged wood in aquatic habitats in north-western Yunnan, China, are introduced in this paper. The introductions are based on morphology and molecular analysis of DNA sequence data. We also recovered two fresh isolates of Dictyocheirospora rotunda D’ Souza, Bhat & K.D. Hyde, which is a first record for China. Descriptions and illustrations of D. aquatica, D. garethjonesii and D. rotunda are provided. The phylogenetic analysis of combined ITS and LSU sequence data place the isolates within the family Dictyosporiaceae (Pleosporales). A synopsis of characters of the accepted species of Dictyocheirospora is provided.

Keywords: Asexual morphs – Dictyosporiaceae – Phylogeny – Taxonomy

10. Neoleptosphaeria jonesii sp. nov., a novel saprobic sexual species, in Leptosphaeriaceae

Authors: Wanasinghe DN, Camporesi E, Hu DM

Recieved: 23 November 2016, Accepted: 10 December 2016, Published: 28 December 2016

Neoleptosphaeria is a genus of ascomycetes known only from its asexual morphs (coelomycetous) and its species have saprobic and / or endophytic life modes. We obtained LSU, SSU and ITS sequence data from a single spore isolation of a freshly collected specimen. A phylogeny of representative strains of the genus and other taxa in Leptosphaeriaceae was obtained. Neoleptosphaeria proved to be strongly monophyletic but related to other genera in Leptosphaeriaceae. Phylogenetic analyses place our new isolate in a strongly supported clade with the generic type of Neoleptosphaeria (N. rubefaciens). The sexual morph of Neoleptosphaeria is therefore established and includes the first genus with muriform ascospores in Leptosphaeriaceae.

Keywords: asexual morph – dictyospores – Italy – phylogeny – taxonomy

11. A new species of Daldinia (Xylariaceae) from the Argentine subtropical montane forest

Authors: Sir EB, Lambert C, Wendt L, Hladki AI, Romero AI, Stadler M

Recieved: 22 October 2016, Accepted: 07 December 2016, Published: 28 December 2016

Based on a combination of morphological, molecular phylogenetic and chemotaxonomic evidence, a new species of Daldinia from Northwest of Argentina is described. Daldinia korfii is morphologically related to D. placentiformis, but differs in having brown vinaceous, KOHextractable pigments and the tissue below the perithecial layer is composed of inconspicuous to conspicuous alternating zones in the new taxon.

Keywords: Ascomycetes – Yungas – secondary metabolites – Xylariales

12. Morpho-molecular characterization and epitypification of Annulatascus velatisporus

Authors: Dayarathne MC, Maharachchikumbura SSN, Phookamsak R, Fryar SC, To-anun C, Jones EBG, AlSadi AM, Zelski SE, Hyde KD

Recieved: 16 November 2016, Accepted: 03 December 2016, Published: 28 December 2016

The holotype of Annulatascus velatisporus, the type species of the genus Annulatascus, which is the core species of Annulatascaceae (Annulatascales) is in poor condition as herbarium material has few ascomata and molecular data could not be generated. Therefore, an epitype is herein designated for the precise delineation of this taxon. A morphological description and DNA characterization are provided for the epitype, obtained from the holotype location. Therefore, this specimen merits use as an interpretive type (epitype) for the holotype of A. velatisporus collected from submerged wood in a river, at Milaa Milaa Falls, north Queensland

Keywords: aquatic fungi – phylogeny – submerged wood – taxonomy – type species

13. The genus Cookeina

Authors: Ekanayaka AH, Hyde KD, Zhao Q

Recieved: 09 November 2016, Accepted: 30 November 2016, Published: 28 December 2016

Cookeina (Sarcoscyphaceae) is characterized by large, brightly coloured, stipitate apothecia and eccentrically, operculate asci. This paper provides detailed morphological descriptions of the sexual morphs of all the recorded species within the genus, including a new species, Cookeina garethjonesii. Phylogenetic analyses inferred from ITS sequence data strongly support species lineages with corresponding morphological differences.

Keywords: apothecia – discomycetes – operculate – phylogeny – taxonomy

14. Characterization of Pisolithus orientalis in culture and in vitro mycorrhization with Eucalyptus camaldulensis and Pinus kesiya

Authors: Kumla J, Suwannarach N, Lumyong S

Recieved: 22 November 2016, Accepted: 15 December 2016, Published: 28 December 2016

A basidiome of Pisolithus orientalis was collected from northern Thailand and a pure culture was isolated. This study aimed to investigate the suitable mycelial growth conditions of this fungus and its ability to form ectomycorrhizal symbiosis with seedlings of Eucalyptus camaldulensis and Pinus kesiya in vitro. The fungal mycelium grew well on Murashige and Skoog medium that was adjusted to pH 5.0 at 30 °C. The plant seedlings were inoculated with fungal mycelium. The mycorrhization was first observed on pine and eucalyptus at 10 and 12 weeks after inoculation, respectively. Moreover, the identification of mycorrhizal association was confirmed by molecular methods. This is the first report of pure culture synthesis of Pis. orientalis mycorrhizae and the compatible of mycorrhizal formation of E. camaldulensis.

Keywords: Ectomycorrhizal fungus – mycelial growth – mycorrhizal synthesis

15. An advance in the endophyte story: Oxydothidaceae fam. nov. with six new species of Oxydothis

Authors: Konta S, Hongsanan S, Tibpromma S, Thongbai B, Maharachchikumbura SSN, Bahkali AH, Hyde KD, Boonmee S

Recieved: 13 November 2016, Accepted: 04 December 2016, Published: 28 December 2016

Oxydothis species are associated with monocotyledons including Arecaceae (palms), Pandanaceae, Poaceae (Bamboo) and Liliaceae and have been recorded as endophytes, pathogens and saprobes. Species of Oxydothis form singly or in clusters, as darkened, raised regions or dots on the surface of host. This paper clarifies the placement of Oxydothis and related species based on morphological characteristics and phylogenetic analyses using recent collections from Thailand. Oxydothis species are characterized by cylindrical asci, with a J+ (rarely J-) subapical ring and filiform to fusiform, hyaline, 1-septate ascospores, tapering from the center to spine-like, pointed or rounded ends. Phylogenetic analyses generated from maximum likelihood and Bayesian analysis of combined ITS, LSU and SSU sequence data indicate that Oxydothis species form a distinct lineage related to Vialaeaceae and Iodosphaeriaceae. We introduce a new family Oxydothidaceae in Xylariales with six new species based on morphological characteristics and phylogenetic analyses. We provided notes on the lifestyle of this genus which may throw light on the nature of many saprobic microfungi.

Keywords: ascomycetes – hyaline ascospores – palm fungi – Xylariales

16. Phylogenetic placement of Neorhamphoria garethjonesii gen. et sp. nov (Tubeufiales, genus, incertae sedis).

Authors: Boonmee S, Ekanayaka AH, Hyde KD, Hüseyin E, Ekici K, Selçuk

Recieved: 19 November 2016, Accepted: 12 December 2016, Published: 28 December 2016

Neorhamphoria garethjonesii is introduced for a new genus and species with apothecial ascomata growing on dead wood of Cotoneaster nummularius (Rosaceae) in terrestrial habitats. Neorhamphoria is characterized by its dark apothecial ascomata, broad cellular pseudoparaphyses, with bitunicate, broad-clavate asci, and hyaline, muriform ascospores. Molecular phylogenetic analyses of combined LSU, SSU and TEF1α sequence data indicate that Neorhamphoria belongs in Dothideomycetes and is related to Tubeufiales. Morphologically, Neorhamphoria differs from all genera of Tubeufiales and thus we treat it as a new genus in Tubeufiales, genus incertae sedis.

Keywords: apothecial ascomata – bitunicate asci – lignicolous, molecular phylogeny – muriform ascospores

17. Neooccultibambusa jonesii, a novel taxon within Occultibambusaceae

Authors: Jayasiri SC, Hyde KD, Jeewon R, Bhat JD, Camporesi E, Kang JC

Recieved: 18 November 2016, Accepted: 23 December 2016, Published: 28 December 2016

A new species of Neooccultibambusa, N. jonesii, is introduced from Ammophila arenaria collected in Italy. The novel taxon is characterized by immersed to semi-immersed ascomata in fissures of bark, a two-layered peridium, hyaline to brown ascospores and in producing chlamydospores in culture; these characters are typical of Neooccultibambusa. Multigene phylogenies based on analysis of 28S, 18S and RPB2 sequence data, indicate that N. jonesii belongs in the family Occultibambusaceae and is closely related to N. chiangraiensis, but is phylogenetically distinct. Neooccultibambusa jonesii can be differentiated from N. chiangraiensis based on the number of septa, the larger and mature dark brown ascospores and in lacking a mucilaginous sheath. We provide a description and photoplates of the new species and compare it with related species, together with phylogenetic inferences based on combined sequence data.

Keywords: chlamydospores – Dothideomycetes – monocotyledons – RPB2–rDNA phylogeny – grass

18. The asexual morph of Trichomerium gloeosporum

Authors: Hongsanan S, Tian Q, Hyde KD, Hu DM

Recieved: 01 November 2016, Accepted: 03 December 2016, Published: 28 December 2016

Trichomerium species are sooty molds that develop superficially on the host surface. There is little morphological and molecular data for the genus. The asexual morphs of Trichomerium have been poorly studied, with also lack molecular data. Several researchers have noted that Tripospermum is possibly the asexual morph of Trichomerium species. In this study, we collected Trichomerium-like taxa from leaves in northern Thailand. The sexual structures found on the specimen were identified as Trichomerium gloeosporum, and Tripospermum-like conidia were also associated with the sexual morph. Cultures were obtained from single Tripospermum-like conidia and sequenced. Phylogenetic analyses generated from maximum likelihood and Bayesian analyses of combined LSU and ITS sequence data demonstrate that our strains isolated from Tripospermumlike conidia clustered with strains of T. gloeosporum with high statistical support. A description of the asexual morph of T. gloeosporum with molecular data is provided.

Keywords: Asexual morph – Trichomeriaceae – sooty molds

19. Muyocopron garethjonesii sp. nov. (Muyocopronales, Dothideomycetes) on Pandanus sp

Authors: Tibpromma S, McKenzie EHC, Karunarathna SC, Xu J Hyde KD, Hu DM

Recieved: 11 November 2016, Accepted: 18 December 2016, Published: 28 December 2016

During a survey of microfungi on leaves of Pandanus species in the Nabanhe Valley at Xishuangbanna, Yunnan Province, China in August 2016, we collected and isolated a new saprobic taxon belonging in Muyocopron (Muyocopronaceae). Both morphological characters and phylogenetic analyses of LSU and SSU sequence data provide strong evidence to place the new taxon in Muyocopron where it is described as M. garethjonesii. A description, photoplates of the macro- and micro-characteristics, and a phylogenetic tree to resolve the placement of M. garethjonesii are provided.

Keywords: Carbonaceous – Pandanaceae – Phylogenetics – Taxonomy